Publications
2023
Van Itallie ES, Field CM, Mitchison TJ, Kirschner MW. 2023. Dorsal lip maturation and initial archenteron extension depend on Wnt11 family ligands.. Dev Biol. 493:67-79. Link to PDF
Gruver S, Rata S, Peshkin L, Kirschner MW. Identification of kinases activated by multiple pro-angiogenic growth factors. Front Pharmacol. 2023 Jan 4;13:1022722. doi: 10.3389/fphar.2022.1022722. Link to PDF
Jonas-Closs RA, Peshkin L. Effective Rapid Blood Perfusion in Xenopus. J Vis Exp. 2023 May 16;(195). doi: 10.3791/65287. PubMed PMID: 37318240; NIHMSID:NIHMS1919837. Link to PDF
Morselli M, Bennett R, Shaidani NI, Horb M, Peshkin L, Pellegrini M. Age-associated DNA methylation changes in Xenopus frogs. Epigenetics. 2023 Dec;18(1):2201517. doi: 10.1080/15592294.2023.2201517. PubMed PMID: 37092296; PubMed Central PMCID: PMC10128463. Link to PDF
2022
Gruver S, Rata S, Peshkin L, Kirschner MW. 2022. Identification of kinases activated by multiple pro-angiogenic growth factors.. Front Pharmacol. 13:1022722.Link to PDF
Cho Y, Jonas-Closs RA, Yampolsky LY, Kirschner MW, Peshkin L. 2022. Intelligent high-throughput intervention testing platform in Daphnia.. Aging Cell. 21(3):e13571. Link to PDF
Anderson CE, Malek MC, Jonas-Closs RA, Cho Y, Peshkin L, Kirschner MW, Yampolsky LY. 2022. Inverse Lansing Effect: Maternal Age and Provisioning Affecting Daughters' Longevity and Male Offspring Production.. Am Nat. 200(5):704-721. Link to PDF
Anderson CE, Ekwudo MN, Jonas-Closs RA, Cho Y, Peshkin L, Kirschner MW, Yampolsky LY. 2022. Lack of age-related respiratory changes in Daphnia.. Biogerontology. 23(1):85-97. Link to PDF
Moshkovsky AR, Kirschner MW. 2022. The nonredundant nature of the Axin2 regulatory network in the canonical Wnt signaling pathway.. Proc Natl Acad Sci U S A. 119(9). Link to PDF
Li F, Rane RV, Luria V, Xiong Z, Chen J, Li Z, Catullo RA, Griffin PC, Schiffer M, Pearce S et al.. 2022. Phylogenomic analyses of the genus Drosophila reveals genomic signals of climate adaptation.. Mol Ecol Resour. 22(4):1559-1581. Link to PDF
Oh S, Lee CH, Yang W, Li A, Mukherjee A, Basan M, Ran C, Yin W, Tabin CJ, Fu D et al.. 2022. Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.. Proc Natl Acad Sci U S A. 119(17):e2117938119. Link to PDF
Liu X, Oh S, Kirschner MW. 2022. The uniformity and stability of cellular mass density in mammalian cell culture.. Front Cell Dev Biol. 10:1017499. Link to PDF
Gruver S, Rata S, Peshkin L, Kirschner MW. Identification of kinases activated by multiple pro-angiogenic growth factors. Front Pharmacol. 2022;13:1022722. doi: 10.3389/fphar.2022.1022722. eCollection 2022. PubMed PMID: 36686695; PubMed Central PMCID: PMC9847502. Link to PDF
2021
Prensner JR, Enache OM, Luria V, Krug K, Clauser KR, Dempster JM, Karger A, Wang L, Stumbraite K, Wang VM et al.. 2021. Noncanonical open reading frames encode functional proteins essential for cancer cell survival.. Nat Biotechnol. 39(6):697-704. Link to PDF
2020
Vonica A, Bhat N, Phan K, Guo J, Iancu L, Weber JA, Karger A, Cain JW, Wang ECE, DeStefano GM et al.. 2020. Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin.. Dev Biol. 464(1):71-87. Link to PDF
Liu X, Oh S, Peshkin L, Kirschner MW. 2020. Computationally enhanced quantitative phase microscopy reveals autonomous oscillations in mammalian cell growth.. Proc Natl Acad Sci U S A. 117(44):27388-27399. Link to PDF
Liu X, Simon JM, Xie H, Hu L, Wang J, Zurlo G, Fan C, Ptacek TS, Herring L, Tan X et al.. 2020. Genome-wide Screening Identifies SFMBT1 as an Oncogenic Driver in Cancer with VHL Loss.. Mol Cell. 77(6):1294-1306.e5. Link to PDF
Ma W, Chen M, Kang H, Steinhart Z, Angers S, He X, Kirschner MW. 2020. Single-molecule dynamics of Dishevelled at the plasma membrane and Wnt pathway activation.. Proc Natl Acad Sci U S A. 117(28):16690-16701. Link to PDF
Mugahid D, Kalocsay M, Liu X, Gruver JScott, Peshkin L, Kirschner MW. 2020. YAP regulates cell size and growth dynamics via non-cell autonomous mediators.. Elife. 9. Link to PDF
Peshkin L, Kirschner MW. 2020. A cell type annotation Jamboree-Revival of а communal science forum. Genesis. Sep;58(9):e23383. doi: 10.1002/dvg.23383. Epub 2020 Jun 25. Link to PDF
Kirschner M.. 2020. What makes the cell cycle tick? a celebration of the awesome power of biochemistry and the frog egg. Mol Biol Cell. ec 15;31(26):2874-2878. doi: 10.1091/mbc.E20-10-0626. Link to PDF
2019
Peshkin L, Gupta M, Ryazanova L, Wühr M. 2019. Bayesian Confidence Intervals for Multiplexed Proteomics Integrate Ion-statistics with Peptide Quantification Concordance.. Mol Cell Proteomics. 18(10):2108-2120. Link to PDF
Korotkova DD, Lyubetsky VA, Ivanova AS, Rubanov LI, Seliverstov AV, Zverkov OA, Martynova NYu, Nesterenko AM, Tereshina MB, Peshkin L et al.. 2019. Bioinformatics Screening of Genes Specific for Well-Regenerating Vertebrates Reveals c-answer, a Regulator of Brain Development and Regeneration.. Cell Rep. 29(4):1027-1040.e6. Link to PDF
Kim H-M, Weber JA, Lee N, Park SGu, Cho YSung, Bhak Y, Lee N, Jeon Y, Jeon S, Luria V et al.. 2019. The genome of the giant Nomura's jellyfish sheds light on the early evolution of active predation.. BMC Biol. 17(1):28. Link to PDF
Peshkin L, Boukhali M, Haas W, Kirschner MW, Yampolsky LY. 2019. Quantitative Proteomics Reveals Remodeling of Protein Repertoire Across Life Phases of Daphnia pulex.. Proteomics. 19(24):e1900155. Link to PDF
2018
Ginzberg MB, Chang N, D'Souza H, Patel N, Kafri R, Kirschner MW. 2018. Cell size sensing in animal cells coordinates anabolic growth rates and cell cycle progression to maintain cell size uniformity. Elife. 11(7). Link to PDF
Briggs JA, Weinreb C, Wagner DE, Megason S, Peshkin L, Kirschner MW, Klein AM. 2018. The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution. Science. 360(6392). Link to PDF
Liu S, Ginzberg MB, Patel N, Hild N, Leung B, Li Z, Chen YC, Chang N, Wang Y, Tan C et al.. 2018. Size uniformity of animal cells is actively maintained by a p38 MAPK-dependent regulation of G1-length. Elife. Link to PDF
Zhang J, Wu T, Simon J, Takada M, Saito R, Fan C, De Liu X-, Jonasch E, Xie L, Chen X et al.. 2018. VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal cell carcinoma.. Science. 361(6399):290-295. Link to PDF
Darras S, Fritzenwanker JH, Uhlinger KR, Farrelly E, Pani AM, Hurley IA, Norris RP, Osovitz M, Terasaki M, Wu M, Aronowicz J, Kirschner M, Gerhart JC, Lowe CJ. 2018. Anteroposterior axis patterning by early canonical Wnt signaling during hemichordate development. PLoS Biol. Jan 16;16(1):e2003698. doi: 10.1371/journal.pbio.2003698. eCollection 2018 Jan.
2017
Wan L, Chen M, Cao J, Dai X, Yin Q, Zhang J, Song S-J, Lu Y, Liu J, Inuzuka H et al.. 2017. The APC/C E3 Ligase Complex Activator FZR1 Restricts BRAF Oncogenic Function.. Cancer Discov. 7(4):424-441. Link to PDF
Lu Y, Wu J, Dong Y, Chen S, Sun S, Ma Y-B, Ouyang Q, Finley D, Kirschner MW, Mao Y. 2017. Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.. Mol Cell. 67(2):322-333.e6. Link to PDF
Gujral TS, Kirschner MW. 2017. Hippo pathway mediates resistance to cytotoxic drugs.. Proc Natl Acad Sci U S A. 114(18):E3729-E3738. Link to PDF
Lee H-J, Jedrychowski MP, Vinayagam A, Wu N, Shyh-Chang N, Hu Y, Min-Wen C, Moore JK, Asara JM, Lyssiotis CA et al.. 2017. Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation.. Cell Rep. 20(3):721-736. Link to PDF
Presler M, Van Itallie E, Klein AM, Kunz R, Coughlin ML, Peshkin L, Gygi SP, Wuhr M, Kirschner MW. 2017. Proteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg. Proc Natl Acad Sci USA. 114(50). Link to PDF
Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantú AV, Choi SS, Jong BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R, Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH. 2017. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. Elife. Sep 8;6:e26509. doi: 10.7554/eLife.26509. Link to PDF
Briggs JA, Li VC, Lee S, Woolf CJ, Klein A, Kirschner MW. 2017. Mouse embryonic stem cells can differentiate via multiple paths to the same state. Elife. Oct 9;6:e26945. doi: 10.7554/eLife.26945. Link to PDF
2016
Brown NG, VanderLinden R, Watson ER, Weissmann F, Ordureau A, Wu K-P, Zhang W, Yu S, Mercredi PY, Harrison JS et al.. 2016. Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.. Cell. 165(6):1440-53. Link to PDF
Hanschen ER, Marriage TN, Ferris PJ, Hamaji T, Toyoda A, Fujiyama A, Neme R, Noguchi H, Minakuchi Y, Suzuki M et al.. 2016. The Gonium pectorale genome demonstrates co-option of cell cycle regulation during the evolution of multicellularity.. Nat Commun. 7:11370. Link to PDF
Berg JM, Bhalla N, Bourne PE, Chalfie M, Drubin DG, Fraser JS, Greider CW, Hendricks M, Jones C, Kiley R et al.. 2016. SCIENTIFIC COMMUNITY. Preprints for the life sciences.. Science. 352(6288):899-901. Link to PDF
Chen S, Wu J, Lu Y, Ma Y-B, Lee B-hoon, Yu Z, Ouyang Q, Finley DJ, Kirschner MW, Mao Y. 2016. Structural basis for dynamic regulation of the human 26S proteasome.. Proc Natl Acad Sci U S A. 113(46):12991-12996. Link to PDF
2015
Ginzberg MB, Kafri R, Kirschner MW. 2015. Cell biology. On being the right (cell) size.. Science. 348(6236):1245075. Link to PDF
Klein AM, Mazutis L, Akartuna I, Tallapragada N, Veres A, Li V, Peshkin L, Weitz DA, Kirschner MW. 2015. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells.. Cell. 161(5):1187-201. Link to PDF
Simakov O, Kawashima T, Marlétaz F, Jenkins J, Koyanagi R, Mitros T, Hisata K, Bredeson J, Shoguchi E, Gyoja F et al.. 2015. Hemichordate genomes and deuterostome origins.. Nature. 527(7579):459-65. Link to PDF
Wühr M, Güttler T, Peshkin L, McAlister GC, Sonnett M, Ishihara K, Groen AC, Presler M, Erickson BK, Mitchison TJ et al.. 2015. The Nuclear Proteome of a Vertebrate.. Curr Biol. 25(20):2663-71. Link to PDF
Alberts B, Kirschner MW, Tilghman S, Varmus H. 2015. Opinion: Addressing systemic problems in the biomedical research enterprise.. Proc Natl Acad Sci U S A. 112(7):1912-3. Link to PDF
Peshkin L, Wühr M, Pearl E, Haas W, Freeman RM, Gerhart JC, Klein AM, Horb M, Gygi SP, Kirschner MW. 2015. On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.. Dev Cell. 35(3):383-94. Link to PDF
Son S, Kang JHo, Oh S, Kirschner MW, Mitchison TJ, Manalis S. 2015. Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis.. J Cell Biol. 211(4):757-63. Link to PDF
Lu Y, Wang W, Kirschner MW. 2015. Specificity of the anaphase-promoting complex: a single-molecule study.. Science. 348(6231):1248737. Link to PDF
Lu Y, Lee B-hoon, King RW, Finley D, Kirschner MW. 2015. Substrate degradation by the proteasome: a single-molecule kinetic analysis.. Science. 348(6231):1250834. Link to PDF
2014
Wan L, Tan M, Yang J, Inuzuka H, Dai X, Wu T, Liu J, Shaik S, Chen G, Deng J et al.. 2014. APC(Cdc20) suppresses apoptosis through targeting Bim for ubiquitination and destruction.. Dev Cell. 29(4):377-91. Link to PDF
Liu P, Begley M, Michowski W, Inuzuka H, Ginzberg M, Gao D, Tsou P, Gan W, Papa A, Kim BMo et al.. 2014. Cell-cycle-regulated activation of Akt kinase by phosphorylation at its carboxyl terminus.. Nature. 508(7497):541-5. Link to PDF
Wühr M, Freeman RM, Presler M, Horb ME, Peshkin L, Gygi SP, Kirschner MW. 2014. Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.. Curr Biol. 24(13):1467-75. Link to PDF
Gujral TSingh, Peshkin L, Kirschner MW. 2014. Exploiting polypharmacology for drug target deconvolution.. Proc Natl Acad Sci U S A. 111(13):5048-53. Link to PDF
Wang W, Wu T, Kirschner MW. 2014. The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium.. Elife. 3:e03083. Link to PDF
Ortiz-Meoz RF, Merbl Y, Kirschner MW, Walker S. 2014. Microarray discovery of new OGT substrates: the medulloblastoma oncogene OTX2 is O-GlcNAcylated.. J Am Chem Soc. 136(13):4845-8. Link to PDF
Li VC, Kirschner MW. 2014. Molecular ties between the cell cycle and differentiation in embryonic stem cells.. Proc Natl Acad Sci U S A. 111(26):9503-8. Link to PDF
Gujral TS, Chan M, Peshkin L, Sorger PK, Kirschner MW, MacBeath G. 2014. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis.. Cell. 159(4):844-56. Link to PDF
Zhao R, Deibler RW, Lerou PH, Ballabeni A, Heffner GC, Cahan P, Unternaehrer JJ, Kirschner MW, Daley GQ. 2014. A nontranscriptional role for Oct4 in the regulation of mitotic entry.. Proc Natl Acad Sci U S A. 111(44):15768-73. Link to PDF
Satoh N, Tagawa K, Lowe CJ, Yu J-K, Kawashima T, Takahashi H, Ogasawara M, Kirschner MW, Hisata K, Su Y-H et al.. 2014. On a possible evolutionary link of the stomochord of hemichordates to pharyngeal organs of chordates.. Genesis. 52(12):925-34. Link to PDF
Merbl Y, Kirschner MW. 2014. Post-Translational Modification Profiling-a High-Content Assay for Identifying Protein Modifications in Mammalian Cellular Systems.. Curr Protoc Protein Sci. 77:27.8.1-27.8.13. Link to PDF
Thompson JW, Nagel J, Hoving S, Gerrits B, Bauer A, Thomas JR, Kirschner MW, Schirle M, Luchansky SJ. 2014. Quantitative Lys-ϵ-Gly-Gly (diGly) proteomics coupled with inducible RNAi reveals ubiquitin-mediated proteolysis of DNA damage-inducible transcript 4 (DDIT4) by the E3 ligase HUWE1.. J Biol Chem. 289(42):28942-55. Link to PDF
Alberts B, Kirschner MW, Tilghman S, Varmus H. 2014. Rescuing US biomedical research from its systemic flaws.. Proc Natl Acad Sci U S A. 111(16):5773-7. Link to PDF
2013
Kirschner MW. 2013. Beyond Darwin: evolvability and the generation of novelty.. BMC Biol. 11:110. Link to PDF
Xie Y, Avello M, Schirle M, McWhinnie E, Feng Y, Bric-Furlong E, Wilson C, Nathans R, Zhang J, Kirschner MW et al.. 2013. Deubiquitinase FAM/USP9X interacts with the E3 ubiquitin ligase SMURF1 protein and protects it from ligase activity-dependent self-degradation.. J Biol Chem. 288(5):2976-85. Link to PDF
Kafri R, Levy J, Ginzberg MB, Oh S, Lahav G, Kirschner MW. 2013. Dynamics extracted from fixed cells reveal feedback linking cell growth to cell cycle.. Nature. 494(7438):480-3. Link to PDF
Wang W, Kirschner MW. 2013. Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex.. Nat Cell Biol. 15(7):797-806. Link to PDF
Ballabeni A, Zamponi R, Moore JK, Helin K, Kirschner MW. 2013. Geminin deploys multiple mechanisms to regulate Cdt1 before cell division thus ensuring the proper execution of DNA replication.. Proc Natl Acad Sci U S A. 110(30):E2848-53. Link to PDF
Cooper KL, Oh S, Sung Y, Dasari RR, Kirschner MW, Tabin CJ. 2013. Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions.. Nature. 495(7441):375-8. Link to PDF
Kirschner MW. 2013. A perverted view of "impact".. Science. 340(6138):1265. Link to PDF
Gallop JL, Walrant A, Cantley LC, Kirschner MW. 2013. Phosphoinositides and membrane curvature switch the mode of actin polymerization via selective recruitment of toca-1 and Snx9.. Proc Natl Acad Sci U S A. 110(18):7193-8. Link to PDF
Merbl Y, Refour P, Patel H, Springer M, Kirschner MW. 2013. Profiling of ubiquitin-like modifications reveals features of mitotic control.. Cell. 152(5):1160-72. Link to PDF
Fukushima H, Ogura K, Wan L, Lu Y, Li V, Gao D, Liu P, Lau AW, Wu T, Kirschner MW et al.. 2013. SCF-mediated Cdh1 degradation defines a negative feedback system that coordinates cell-cycle progression.. Cell Rep. 4(4):803-16. Link to PDF
Sung Y, Tzur A, Oh S, Choi W, Li V, Dasari RR, Yaqoob Z, Kirschner MW. 2013. Size homeostasis in adherent cells studied by synthetic phase microscopy.. Proc Natl Acad Sci U S A. 110(41):16687-92. Link to PDF
2012
Wühr M, Haas W, McAlister GC, Peshkin L, Rad R, Kirschner MW, Gygi SP. 2012. Accurate multiplexed proteomics at the MS2 level using the complement reporter ion cluster. Anal Chem. 84(21):9214-21. Link to PDF
Son S, Tzur A, Weng Y, Jorgensen P, Kim J, Kirschner MW, Manalis SR. 2012. Direct observation of mammalian cell growth and size regulation. Nat Methods. 9(9):910-2. Link to PDF
Li VC, Ballabeni A, Kirschner MW. 2012. Gap 1 phase length and mouse embryonic stem cell self-renewal. Proc Natl Acad Sci U S A. 109(31):12550-5. Link to PDF
Freeman R, Ikuta T, Wu M, Koyanagi R, Kawashima T, Tagawa K, Humphreys T, Fang G-C, Fujiyama A, Saiga H et al.. 2012. Identical genomic organization of two hemichordate hox clusters. Curr Biol. 22(21):2053-8. Link to PDF
Hernández AR, Klein AM, Kirschner MW. 2012. Kinetic responses of β-catenin specify the sites of Wnt control. Science. 338(6112):1337-40. Link to PDF
Jain M, Nilsson R, Sharma S, Madhusudhan N, Kitami T, Souza AL, Kafri R, Kirschner MW, Clish CB, Mootha VK. 2012. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 336(6084):1040-4. Link to PDF
Hao H-X, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H et al.. 2012. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 485(7397):195-200. Link to PDF
2011
Ballabeni A, Park I-H, Zhao R, Wang W, Lerou PH, Daley GQ, Kirschner MW. 2011. Cell cycle adaptations of embryonic stem cells. Proc Natl Acad Sci U S A. 108(48):19252-7. Link to PDF
Ali F, Hindley C, McDowell G, Deibler R, Jones A, Kirschner MW, Guillemot F, Philpott A. 2011. Cell cycle-regulated multi-site phosphorylation of Neurogenin 2 coordinates cell cycling with differentiation during neurogenesis. Development. 138(19):4267-77. Link to PDF
Kirschner MW, Shapiro L, McAdams H, Almouzni G, Sharp PA, Young RA, Alon U. 2011. Fifty years after Jacob and Monod: what are the unanswered questions in molecular biology? Mol Cell. 42(4):403-4. Link to PDF
Yanai I, Peshkin L, Jorgensen P, Kirschner MW. 2011. Mapping gene expression in two Xenopus species: evolutionary constraints and developmental flexibility. Dev Cell. 20(4):483-96. Link to PDF
Kirschner MW. 2011. Marc Kirschner. Interview by Asher Mullard. Nat Rev Drug Discov. 10(12):894. Link to PDF
Tzur A, Moore JK, Jorgensen P, Shapiro HM, Kirschner MW. 2011. Optimizing optical flow cytometry for cell volume-based sorting and analysis. PLoS One. 6(1):e16053. Link to PDF
Merbl Y, Kirschner MW. 2011. Protein microarrays for genome-wide posttranslational modification analysis. Wiley Interdiscip Rev Syst Biol Med. 3(3):347-56. Link to PDF
Vastag L, Jorgensen P, Peshkin L, Wei R, Rabinowitz JD, Kirschner MW. 2011. Remodeling of the metabolome during early frog development. PLoS One. 6(2):e16881. Link to PDF
Darras S, Gerhart J, Terasaki M, Kirschner MW, Lowe CJ. 2011. β-catenin specifies the endomesoderm and defines the posterior organizer of the hemichordate Saccoglossus kowalevskii. Development. 138(5):959-70. Link to PDF
2010
Kirschner MW. 2010. Cell biology as a world view. Mol Biol Cell. 21(22):3803. Link to PDF
Wu S, Wang W, Kong X, Congdon LM, Yokomori K, Kirschner MW, Rice JC. 2010. Dynamic regulation of the PR-Set7 histone methyltransferase is required for normal cell cycle progression. Genes Dev. 24(22):2531-42. Link to PDF
Springer M, Weissman JS, Kirschner MW. 2010. A general lack of compensation for gene dosage in yeast. Mol Syst Biol. 6:368. Link to PDF
DeLuna A, Springer M, Kirschner MW, Kishony R. 2010. Need-based up-regulation of protein levels in response to deletion of their duplicate genes. PLoS Biol. 8(3):e1000347. Link to PDF
Song EJoo, Werner SL, Neubauer J, Stegmeier F, Aspden J, Rio D, J Harper W, Elledge SJ, Kirschner MW, Rape M. 2010. The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome. Genes Dev. 24(13):1434-47. Link to PDF
Deibler RW, Kirschner MW. 2010. Quantitative reconstitution of mitotic CDK1 activation in somatic cell extracts. Mol Cell. 37(6):753-67. Link to PDF
Lee K, Gallop JL, Rambani K, Kirschner MW. 2010. Self-assembly of filopodia-like structures on supported lipid bilayers. Science. 329(5997):1341-5. Link to PDF
Wu T, Merbl Y, Huo Y, Gallop JL, Tzur A, Kirschner MW. 2010. UBE2S drives elongation of K11-linked ubiquitin chains by the anaphase-promoting complex. Proc Natl Acad Sci U S A. 107(4):1355-60. Link to PDF
Godin M, Delgado FFeijó, Son S, Grover WH, Bryan AK, Tzur A, Jorgensen P, Payer K, Grossman AD, Kirschner MW et al.. 2010. Using buoyant mass to measure the growth of single cells. Nat Methods. 7(5):387-90. Link to PDF
2009
Lebensohn AM, Kirschner MW. 2009. Activation of the WAVE complex by coincident signals controls actin assembly. Mol Cell. 36(3):512-24. Link to PDF
Gao D, Inuzuka H, Korenjak M, Tseng A, Wu T, Wan L, Kirschner MW, Dyson N, Wei W. 2009. Cdh1 regulates cell cycle through modulating the claspin/Chk1 and the Rb/E2F1 pathways. Mol Biol Cell. 20(14):3305-16. Link to PDF
Tzur A, Kafri R, LeBleu VS, Lahav G, Kirschner MW. 2009. Cell growth and size homeostasis in proliferating animal cells. Science. 325(5937):167-71. Link to PDF
Goentoro L, Kirschner MW. 2009. Evidence that fold-change, and not absolute level, of beta-catenin dictates Wnt signaling. Mol Cell. 36(5):872-84. Link to PDF
Singh S, Springer M, Steen J, Kirschner MW, Steen H. 2009. FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res. 8(5):2201-10. Link to PDF
Goentoro L, Shoval O, Kirschner MW, Alon U. 2009. The incoherent feedforward loop can provide fold-change detection in gene regulation. Mol Cell. 36(5):894-9. Link to PDF
Merbl Y, Kirschner MW. 2009. Large-scale detection of ubiquitination substrates using cell extracts and protein microarrays. Proc Natl Acad Sci U S A. 106(8):2543-8. Link to PDF
Jorgensen P, Steen JAJ, Steen H, Kirschner MW. 2009. The mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus. Development. 136(9):1539-48. Link to PDF
Huang S-MA, Mishina YM, Liu S, Cheung A, Stegmeier F, Michaud GA, Charlat O, Wiellette E, Zhang Y, Wiessner S et al.. 2009. Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature. 461(7264):614-20. Link to PDF
2008
Freeman RM, Wu M, Cordonnier-Pratt M-M, Pratt LH, Gruber CE, Smith M, Lander ES, Stange-Thomann N, Lowe CJ, Gerhart J et al.. 2008. cDNA sequences for transcription factors and signaling proteins of the hemichordate Saccoglossus kowalevskii: efficacy of the expressed sequence tag (EST) approach for evolutionary and developmental studies of a new organism. Biol Bull. 214(3):284-302. Link to PDF
Steen JAJ, Steen H, Georgi A, Parker K, Springer M, Hamprecht F, Kirschner MW. 2008. Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A. 105(16):6069-74. Link to PDF
Kirschner MW. 2008. Profile: Bruce Alberts, Science's new editor. Science. 319(5867):1199. Link to PDF
2007
Weiner OD, Marganski WA, Wu LF, Altschuler SJ, Kirschner MW. 2007. An actin-based wave generator organizes cell motility. PLoS Biol. 5(9):e221. Link to PDF
Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, E McDonald R, Li MZ, Hannon GJ, Sorger PK et al.. 2007. Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities. Nature. 446(7138):876-81. Link to PDF
Rickmyre JL, Dasgupta S, Ooi DLiang-Yee, Keel J, Lee E, Kirschner MW, Waddell S, Lee LA. 2007. The Drosophila homolog of MCPH1, a human microcephaly gene, is required for genomic stability in the early embryo. J Cell Sci. 120(Pt 20):3565-77. Link to PDF
Wang R, He G, Nelman-Gonzalez M, Ashorn CL, Gallick GE, P Stukenberg T, Kirschner MW, Kuang J. 2007. Regulation of Cdc25C by ERK-MAP kinases during the G2/M transition. Cell. 128(6):1119-32. Link to PDF
Milo R, Hou JH, Springer M, Brenner MP, Kirschner MW. 2007. The relationship between evolutionary and physiological variation in hemoglobin. Proc Natl Acad Sci U S A. 104(43):16998-7003. Link to PDF
Gerhart J, Kirschner MW. 2007. The theory of facilitated variation. Proc Natl Acad Sci U S A. 104 Suppl 1:8582-9. Link to PDF
Reddy SK, Rape M, Margansky WA, Kirschner MW. 2007. Ubiquitination by the anaphase-promoting complex drives spindle checkpoint inactivation. Nature. 446(7138):921-5. Link to PDF
2006
Peterson JR, Lebensohn AM, Pelish HE, Kirschner MW. 2006. Biochemical suppression of small-molecule inhibitors: a strategy to identify inhibitor targets and signaling pathway components. Chem Biol. 13(4):443-52. Link to PDF
Lebensohn AM, Ma L, Ho H-YHenry, Kirschner MW. 2006. Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts. Methods Enzymol. 406:156-73. Link to PDF
Bourlat SJ, Juliusdottir T, Lowe CJ, Freeman R, Aronowicz J, Kirschner MW, Lander ES, Thorndyke M, Nakano H, Kohn AB et al.. 2006. Deuterostome phylogeny reveals monophyletic chordates and the new phylum Xenoturbellida. Nature. 444(7115):85-8. Link to PDF
Lowe CJ, Terasaki M, Wu M, Freeman RM, Runft L, Kwan K, Haigo S, Aronowicz J, Lander E, Gruber C et al.. 2006. Dorsoventral patterning in hemichordates: insights into early chordate evolution. PLoS Biol. 4(9):e291. Link to PDF
Weiner OD, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. 2006. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. PLoS Biol. 4(2):e38. Link to PDF
Ho H-YHenry, Rohatgi R, Lebensohn AM, Kirschner MW. 2006. In vitro reconstitution of cdc42-mediated actin assembly using purified components. Methods Enzymol. 406:174-90. Link to PDF
Kirschner MW. 2006. Obituary: Reinhart Heinrich (1946-2006). Nature. 444(7120):700. Link to PDF
Steen H, Jebanathirajah JA, Rush J, Morrice N, Kirschner MW. 2006. Phosphorylation analysis by mass spectrometry: myths, facts, and the consequences for qualitative and quantitative measurements. Mol Cell Proteomics. 5(1):172-81. Link to PDF
Rape M, Reddy SK, Kirschner MW. 2006. Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Cell. 124(1):89-103. Link to PDF
2005
Jebanathirajah JA, Pittman JL, Thomson BA, Budnik BA, Kaur P, Rape M, Kirschner MW, Costello CE, O'Connor PB. 2005. Characterization of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of whole proteins. J Am Soc Mass Spectrom. 16(12):1985-99. Link to PDF
Viadiu H, Stemmann O, Kirschner MW, Walz T. 2005. Domain structure of separase and its binding to securin as determined by EM. Nat Struct Mol Biol. 12(6):552-3. Link to PDF
Lee LA, Lee E, Anderson MA, Vardy L, Tahinci E, Ali SM, Kashevsky H, Benasutti M, Kirschner MW, Orr-Weaver TL. 2005. Drosophila genome-scale screen for PAN GU kinase substrates identifies Mat89Bb as a cell cycle regulator. Dev Cell. 8(3):435-42. Link to PDF
Gerhart J, Lowe C, Kirschner MW. 2005. Hemichordates and the origin of chordates. Curr Opin Genet Dev. 15(4):461-7. Link to PDF
Ayad NG, Rankin S, Ooi D, Rape M, Kirschner MW. 2005. Identification of ubiquitin ligase substrates by in vitro expression cloning. Methods Enzymol. 399:404-14. Link to PDF
Kirschner MW. 2005. The meaning of systems biology. Cell. 121(4):503-4. Link to PDF
Kwan KM, Kirschner MW. 2005. A microtubule-binding Rho-GEF controls cell morphology during convergent extension of Xenopus laevis. Development. 132(20):4599-610. Link to PDF
Rankin S, Ayad NG, Kirschner MW. 2005. Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates. Mol Cell. 18(2):185-200. Link to PDF
Steen H, Jebanathirajah JA, Springer M, Kirschner MW. 2005. Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci U S A. 102(11):3948-53. Link to PDF
2004
Rape M, Kirschner MW. 2004. Autonomous regulation of the anaphase-promoting complex couples mitosis to S-phase entry. Nature. 432(7017):588-95. Link to PDF
Peterson JR, Bickford LC, Morgan D, Kim AS, Ouerfelli O, Kirschner MW, Rosen MK. 2004. Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat Struct Mol Biol. 11(8):747-55. Link to PDF
Campellone KG, Rankin S, Pawson T, Kirschner MW, Tipper DJ, Leong JM. 2004. Clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly. J Cell Biol. 164(3):407-16. Link to PDF
Wei W, Ayad NG, Wan Y, Zhang G-J, Kirschner MW, Kaelin WG. 2004. Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase-promoting complex. Nature. 428(6979):194-8. Link to PDF
Martinez-Quiles N, Ho H-YHenry, Kirschner MW, Ramesh N, Geha RS. 2004. Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP. Mol Cell Biol. 24(12):5269-80. Link to PDF
Lowe CJ, Tagawa K, Humphreys T, Kirschner MW, Gerhart J. 2004. Hemichordate embryos: procurement, culture, and basic methods. Methods Cell Biol. 74:171-94. Link t0 PDF
Gautreau A, Ho H-yiH, Li J, Steen H, Gygi SP, Kirschner MW. 2004. Purification and architecture of the ubiquitous Wave complex. Proc Natl Acad Sci U S A. 101(13):4379-83. Link to PDF
Margalit DN, Romberg L, Mets RB, Hebert AM, Mitchison TJ, Kirschner MW, RayChaudhuri D. 2004. Targeting cell division: small-molecule inhibitors of FtsZ GTPase perturb cytokinetic ring assembly and induce bacterial lethality. Proc Natl Acad Sci U S A. 101(32):11821-6. Link to PDF
Ho H-YHenry, Rohatgi R, Lebensohn AM, Li J, Gygi SP, Kirschner MW. 2004. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell. 118(2):203-16. Link to PDF
Song MSup, Song SJeong, Ayad NG, Chang JSook, Lee JHyun, Hong HKyung, Lee H, Choi N, Kim J, Kim H et al.. 2004. The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex. Nat Cell Biol. 6(2):129-37. Link to PDF
2003
Gerber SA, Rush J, Stemman O, Kirschner MW, Gygi SP. 2003. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc Natl Acad Sci U S A. 100(12):6940-5. Link to PDF
Lowe CJ, Wu M, Salic A, Evans L, Lander E, Stange-Thomann N, Gruber CE, Gerhart J, Kirschner MW. 2003. Anteroposterior patterning in hemichordates and the origins of the chordate nervous system. Cell. 113(7):853-65. Link to PDF
Osterfield M, Kirschner MW, Flanagan JG. 2003. Graded positional information: interpretation for both fate and guidance. Cell. 113(4):425-8. Link to PDF
Chabes ALena, Pfleger CM, Kirschner MW, Thelander L. 2003. Mouse ribonucleotide reductase R2 protein: a new target for anaphase-promoting complex-Cdh1-mediated proteolysis. Proc Natl Acad Sci U S A. 100(7):3925-9. Link to PDF
Lee E, Salic A, Krüger R, Heinrich R, Kirschner MW. 2003. The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway. PLoS Biol. 1(1):E10. Link to PDF
Collavin L, Kirschner MW. 2003. The secreted Frizzled-related protein Sizzled functions as a negative feedback regulator of extreme ventral mesoderm. Development. 130(4):805-16. Link to PDF
Ayad NG, Rankin S, Murakami M, Jebanathirajah J, Gygi S, Kirschner MW. 2003. Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC. Cell. 113(1):101-13. Link to PDF
Yan C, Martinez-Quiles N, Eden S, Shibata T, Takeshima F, Shinkura R, Fujiwara Y, Bronson R, Snapper SB, Kirschner MW et al.. 2003. WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility. EMBO J. 22(14):3602-12. Link to PDF
Kwan KM, Kirschner MW. 2003. Xbra functions as a switch between cell migration and convergent extension in the Xenopus gastrula. Development. 130(9):1961-72. Link to PDF
2002
Zou H, Stemman O, Anderson JS, Mann M, Kirschner MW. 2002. Anaphase specific auto-cleavage of separase. FEBS Lett. 528(1-3):246-50. Link to PDF
Farhana L, Dawson M, Rishi AK, Zhang Y, Van Buren E, Trivedi C, Reichert U, Fang G, Kirschner MW, Fontana JA. 2002. Cyclin B and E2F-1 expression in prostate carcinoma cells treated with the novel retinoid CD437 are regulated by the ubiquitin-mediated pathway. Cancer Res. 62(13):3842-9. Link to PDF
Castiglione F, Bernaschi M, Succi S, Heinrich R, Kirschner MW. 2002. Intracellular signal propagation in a two-dimensional autocatalytic reaction model. Phys Rev E Stat Nonlin Soft Matter Phys. 66(3 Pt 1):031905. Link to PDF
Eden S, Rohatgi R, Podtelejnikov AV, Mann M, Kirschner MW. 2002. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature. 418(6899):790-3. Link to PDF
Holmen SL, Salic A, Zylstra CR, Kirschner MW, Williams BO. 2002. A novel set of Wnt-Frizzled fusion proteins identifies receptor components that activate beta -catenin-dependent signaling. J Biol Chem. 277(38):34727-35. Link to PDF
Weiner OD, Neilsen PO, Prestwich GD, Kirschner MW, Cantley LC, Bourne HR. 2002. A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity. Nat Cell Biol. 4(7):509-13. Link to PDF
Zhu Z, Kirschner MW. 2002. Regulated proteolysis of Xom mediates dorsoventral pattern formation during early Xenopus development. Dev Cell. 3(4):557-68. Link to PDF
Georgi AB, P Stukenberg T, Kirschner MW. 2002. Timing of events in mitosis. Curr Biol. 12(2):105-14. Link to PDF
2001
Wan Y, Liu X, Kirschner MW. 2001. The anaphase-promoting complex mediates TGF-beta signaling by targeting SnoN for destruction. Mol Cell. 8(5):1027-39. Link to PDF
Peterson JR, Lokey RS, Mitchison TJ, Kirschner MW. 2001. A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions. Proc Natl Acad Sci U S A. 98(19):10624-9. Link to PDF
Ho HY, Rohatgi R, Ma L, Kirschner MW. 2001. CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein family. Proc Natl Acad Sci U S A. 98(20):11306-11. Link to PDF
Stemmann O, Zou H, Gerber SA, Gygi SP, Kirschner MW. 2001. Dual inhibition of sister chromatid separation at metaphase. Cell. 107(6):715-26. Link to PDF
Wan Y, Kirschner MW. 2001. Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities. Proc Natl Acad Sci U S A. 98(23):13066-71. Link to PDF
Roberts RJ, Varmus HE, Ashburner M, Brown PO, Eisen MB, Khosla C, Kirschner MW, Nusse R, Scott M, Wold B. 2001. Information access. Building a "GenBank" of the published literature.. Science. 291(5512):2318-9.
Pfleger CM, Salic A, Lee E, Kirschner MW. 2001. Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1. Genes Dev. 15(14):1759-64. Link to PDF
Davis RL, Turner DL, Evans LM, Kirschner MW. 2001. Molecular targets of vertebrate segmentation: two mechanisms control segmental expression of Xenopus hairy2 during somite formation. Dev Cell. 1(4):553-65. Link to PDF
Rohatgi R, Nollau P, Ho HY, Kirschner MW, Mayer BJ. 2001. Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway. J Biol Chem. 276(28):26448-52. Link to PDF
Lee E, Salic A, Kirschner MW. 2001. Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon. J Cell Biol. 154(5):983-93. Link to PDF
Stukenberg PT, Kirschner MW. 2001. Pin1 acts catalytically to promote a conformational change in Cdc25. Mol Cell. 7(5):1071-83. Link to PDF
Pfleger CM, Lee E, Kirschner MW. 2001. Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex. Genes Dev. 15(18):2396-407. Link to PDF
2000
Chen F, Ma L, Parrini MC, Mao X, Lopez M, Wu C, Marks PW, Davidson L, Kwiatkowski DJ, Kirchhausen T et al.. 2000. Cdc42 is required for PIP(2)-induced actin polymerization and early development but not for cell viability. Curr Biol. 10(13):758-65. Link to PDF
Salic A, Lee E, Mayer L, Kirschner MW. 2000. Control of beta-catenin stability: reconstitution of the cytoplasmic steps of the wnt pathway in Xenopus egg extracts. Mol Cell. 5(3):523-32. Link to PDF
Davis RL, Kirschner MW. 2000. The fate of cells in the tailbud of Xenopus laevis. Development. 127(2):255-67. Link to PDF
Pfleger CM, Kirschner MW. 2000. The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1. Genes Dev. 14(6):655-65. Link to PDF
Feng Y, Olson EC, Stukenberg PT, Flanagan LA, Kirschner MW, Walsh CA. 2000. LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. Neuron. 28(3):665-79. Link to PDF
Rohatgi R, Ho HY, Kirschner MW. 2000. Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate. J Cell Biol. 150(6):1299-310. Link to PDF
Kirschner MW, Gerhart J, Mitchison T. 2000. Molecular "vitalism". Cell. 100(1):79-88. Link to PDF
Luo X, Fang G, Coldiron M, Lin Y, Yu H, Kirschner MW, Wagner G. 2000. Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20.. Nat Struct Biol. 7(3):224-9. Link to PDF
Yamaguchi H, Miki H, Suetsugu S, Ma L, Kirschner MW, Takenawa T. 2000. Two tandem verprolin homology domains are necessary for a strong activation of Arp2/3 complex-induced actin polymerization and induction of microspike formation by N-WASP.. Proc Natl Acad Sci U S A. 97(23):12631-6. Link to PDF